© Copyright 2022 Gator Bio
Vivienne Lee, Indrani Chakraborty, Harsha Agarwal, Wai Choi, Samuel Yang, Katie Trieu, Pu Li, Robert Zuk Gator Bio, Inc, Palo Alto, CA
Introduction
Biolayer Interferometry (BLI) is a label-free analytical technique based on optical interference. An additional layer of biomolecules modifies the phase of light as it passes through. This generates an interference pattern that provides real-time information on binding affinity and interaction kinetics.
Ensuring the quality and consistency of vectors is critical to the success of gene therapy, but several challenges remain, including:
- Accurate titer, % full and partial determination in crude samples
- Use of multiple techniques to characterize viral and lipid particles
- Easy rank ordering of titers and % full during purification in manufacturing
- Highly accurate and precise
- Compatible with various matrices
- Minimal hands-on time
- Can be easily implemented from harvest to final product titer and % full QC
AAV Analytics
AAV CAPSID TITER
AAVX probe and HS AAV kit capture various serotypes of AAV with a dynamic range of 1E+07 to 1E+13 vp/mL.
CRUDE SAMPLE TITER USING HIGH SENSITIVITY AAV KIT
PURIFIED SAMPLE TITER USING AAVX PROBES
DETERMINATION OF AAV EMPTY TO FULL RATIOS
AAV Ratio Kit determines the AAV empty to full ratios through AAV capture, lysis and DNA quantitation. This format works for both crude and purified samples.
DETERMINATION OF AAV EMPTY TO FULL RATIOS
Analyzing Non-Viral Vectors
KINETICS OF PEGYLATED LIPID NANOPARTICLES
Anti-PEG probe captures and quantifies lipid nanoparticles (LNPs). Serum proteins can also be immobilized onto probe to study their kinetic interactions with LNPs.
VIRAL-LIKE PARTICLES
Probes are customized to target surface markers, such as biotin or flag tag, to capture virallike particles (VLPs). The immobilized VLPs can then be used for screening antibodies or ligands against biomolecules embedded in the VLPs.
Summary
The BLI-based solutions address the challenges faced in vector production:
- Accurate, precise and wide dynamic range in crude and clean matrices
- Accurate empty to full ratios enabling rank ordering during purification
- All AAV assays – compatible with various matrices
- Accurate and easy kinetics platform for LNPs and VLPs