BLI Technology Advanced label-free detection Click to Play Video Your browser does not support the video tag. Biolayer Interferometry (BLI) is an advanced optical biosensing technique that enables real-time, label-free analysis of biomolecular interactions. By measuring changes in the interference pattern of white light reflected from a biosensor surface, BLI provides insights into binding events, including association and dissociation rates, without the need for fluorescent or radioactive labels. OUR HISTORY Origins and Evolution of BLI Biolayer interferometry (BLI) was born from the need for a faster, simpler, and label-free way to study biomolecular interactions. Traditional methods were often complex, time-consuming, or limited in flexibility—leaving researchers searching for better solutions. In response, a new approach emerged: BLI provided real-time insights into binding events with unprecedented ease and versatility. Today, BLI continues to evolve, driven by a commitment to innovation that meets the growing demands of modern research—from antibodies to emerging modalities—helping scientists bring greater meaning to every molecule. Explore Instruments Applications in Life Sciences BLI has become an indispensable tool across multiple domains within the life sciences: Drug Discovery and Development BLI facilitates the screening of compound libraries, analysis of structure-activity relationships, and assessment of drug-target interactions. Its real-time monitoring capabilities allow for the evaluation of binding kinetics and affinities, streamlining the drug development process. Protein-Protein and Protein-DNA Interactions Researchers utilize BLI to investigate complex biological processes by studying interactions between proteins or between proteins and nucleic acids. This aids in understanding mechanisms underlying gene regulation, signal transduction, and other cellular functions. Small Molecule Analysis BLI’s sensitivity to molecular binding events makes it suitable for analyzing small molecules, which are often challenging to study due to their low molecular weight. This is particularly valuable in pharmacology, where understanding small molecule interactions is crucial. Biopharmaceutical Characterization In the production of biologics, BLI assists in quality control by characterizing binding properties, ensuring consistency and efficacy of therapeutic proteins. Advantages of BLI BLI offers several benefits that enhance its utility in research and development: Label-Free Detection Eliminates the need for secondary labels, reducing potential interference and simplifying assay design. Real-Time Monitoring Provides immediate insights into binding events, allowing for dynamic analysis of interaction kinetics. High Throughput The “dip-and-read” format enables simultaneous analysis of multiple samples, increasing efficiency in experimental workflows. Versatility Capable of analyzing a wide range of biomolecules, including proteins, nucleic acids, and small molecules, in various sample types, including crude extracts. Minimal Sample Requirement Requires only small volumes of sample, conserving valuable or limited materials. Applications One platform. Endless possibilities. From drug discovery and vaccine development to antibody characterization and quality control, Gator® Bio’s versatile BLI platform supports a wide range of applications across biopharma, academia, and diagnostics. Antibody EngineeringAntibody Drug Conjugates (ADC) Bispecific Antibodies Fc Receptor & C1q Optimization Glycosylation Profiling Neutralizing Antibodies Binder DiscoveryAI/ML-Driven Binder Design Cell Free Expression Analytics Clone Selection Epitope binning and mapping High Throughput Characterization Phage and Yeast display platform Emerging TechnologyAptamer Screening Cell-Free Expression Analytics LNP & EV Characterization Small Molecule & Peptide Biologics ManufacturingActivity & Potency Biologics Quantitation Cell Culture Optimization Formulation & Stability Testing Media Optimization Quality Control & CQAs Cell & Gene TherapyGenome Analysis & AAV % Full CAR-T Therapeutics Development Vaccine Development Viral Vector Titer Previous Next Looking Ahead Impact on Life Sciences The integration of BLI into research and development pipelines has significantly accelerated scientific discovery. Its ability to provide rapid, accurate, and detailed information about molecular interactions has transformed approaches in drug development, molecular biology, and biochemistry. By offering a user-friendly and efficient platform, BLI continues to empower scientists to unravel complex biological systems and develop innovative therapeutic solutions. Advancing the Future of BLI At Gator Bio, we’re reimagining biolayer interferometry (BLI) to empower researchers and accelerate discovery. Building on the simplicity and speed that set BLI apart from traditional methods, we are pushing the boundaries of what’s possible with next-generation innovations. From seamless integration with lab automation for high-throughput screening to AI-driven biosensor design, our solutions are crafted to meet the evolving needs of modern science. Everything we do is guided by a single purpose: to help researchers unlock new insights, overcome challenges, and bring greater meaning to every molecule. About Gator Bio WHAT OUR CUSTOMERS SAY “This was by far the best experience we’ve had with a vendor demo. Having an instrument delivered for hands-on experience was extremely valuable in helping us gain confidence the system could be easily integrated into our characterization processes.” Matt Cummings, PhD Sr. Director, Analytical Sciences, Myris Therapeutics “The Gator is now our lab’s most-used instrument. Its durability and versatility let us screen hundreds of samples, improving antigen affinity five- to six-fold. Compared to other systems, it offers a streamlined workflow, excels in high-throughput screening, and remains highly cost-effective, making it indispensable for our research.” Lei Chen CEO and Founder of Yikang Biotech (Suzhou) “We have an antibody engineering department that reviews molecular candidates to generate different lead molecules. In terms of selecting BLI technology, it’s a really useful tool to screen [molecular] candidates and quickly create a selection to move forward. It’s a really useful tool for me and us.” Kyu Hong Associate director, bioanalysis Immune-Onc Therapeutics “The Gator has been a staple in our lab for six years. Its intuitive software, reliable data, and excellent value set it apart from Biacore and Octet. Ideal for quantitative antibody screening, affinity, and epitope analysis, its wide range of probes provides the flexibility needed for diverse experiments.” Louis Zhang Senior Scientist with over a decade of expertise in antibody discovery and development “…customer service was among the main reasons we chose to work with Gator as opposed to a competitor’s instrument, and I’m glad to see we were right in doing so!” Senior Scientist, Pharmaceutical Company “[Gator Bio] was extremely helpful, flexible, and professional, allowing onsite testing and extended instrument use for rigorous comparison with our existing BLI platform. [The FAS] impressed us with his knowledge and interpersonal skills – rare among vendor FAS. Our experience with Gator Bio has been far more positive, knowledgeable, and supportive than with [their] closest competitor.” Mauro Acchione, PhD Team Leader, Quality Attributes and Characterization, Human Health Therapeutics Research Center, National Research Council Canada Previous Next