Resources Optimizing BLI Assays: Effective Strategies for Reducing Non-Specific Binding Download our new Non-Specific Binding (NSB) Application Note Get the Complete NSB Optimization Technical Documentation Why Download These?Struggling with signal artifacts in complex samples? The NSB Application Note shows you how systematic blocking strategies deliver:✓ 90% reduction in non-specific binding✓ 10x higher data reliability in crude lysates✓ Accurate kinetics for low-concentration analytes✓ Optimized protocols for BSA, detergents, and biocytin✓ Clearer differentiation between true binding and noise Inside you’ll find:– Systematic NSB identification flowchart– Step-by-step optimization protocols– Performance data in cell culture supernatants– Technical troubleshooting guide Complete the form below to access the document
Resources HIS XT Probe: Advanced His-Tag Capture for Kinetics, Quantitation, and Epitope Binning Download our new Anti-His (HIS) XT Probes Application Note and Product Note Get the Complete HIS XT Technical Documentation Why Download These?Struggling with baseline drift in His-tag assays? The HIS XT Application Note shows you how AI-designed NovoBody™ probes deliver:✓ 58% reduction in baseline drift✓ 4x higher signal resolution✓ Cleaner kinetics data for weak binders✓ Extended probe lifetime (10+ regeneration cycles)✓ Zero Fc interference Inside you’ll find:– Performance comparison data– Application protocols (kinetics, quantitation, epitope binning)– Customer validation results– Technical specifications Complete the form below to access the documents
Resources Selection guide for biosensors that bind his-tagged proteins Selection Guide: Gator Probes for His-tagged Protein Capture Gator Bio offers three His-tag capture chemistries, each optimized for different experimental requirements. Selection depends on sample matrix complexity, assay needs, buffer compatibility, and analyte characteristics. Download PDF Recommendations based on sample characteristics: Sample/Assay CharacteristicsAnti-His(PN: 160009)Ni-NTA(PN: 160016)Anti-His (HIS) XT(PN: 160050) Crude samples or complex matrices ✔✔Buffers with reducing agents or chelators✔✔Picomolar affinity measurements✔Small analytes (< 15 kDa) or weak interactions✔Analysis of Fc receptor binding✔✔Sample-limited applications✔Standardized, high-throughput workflows in clean buffers✔✔Table 1: Recommended His-capture probes for common sample and assay needs FeatureAnti-His (Cat# 160009)Ni-NTA (Cat# 160016)Anti-His (HIS) XT (Cat# 160050)Capture SurfaceMonoclonal AntibodyNi-NTA CoordinationAI-Designed NovoBodyTMApplicationsQ / K / EPQ / K / EPQ / K / EPQuantitation Dynamic Range0.25 – 500 µg/ml 0.1 -1000 µg/ml 0.2 – 1000 µg/ml Sample RequirementsHighModerateLowCompatible Sample MatrixPurified proteins E coli lysate CHO Lysate Expi293 Lysate Purified proteins Purified proteins E coli lysate CHO lysate Expi293 lysate Regeneration / Reuse5-10 regenerations 10+ regenerations10+ regenerations Key StrengthsSpecific His-tag recognition; compatible with many buffers Simple, widely used capture chemistry; broad dynamic range Higher signal response per binding event, excellent ligand affinity, low ligand use, optimized to reduce avidity Table 1: Feature comparison between Gator Bio Anti-His, Ni-NTA, and Anti-His (HIS) XT probes. Questions? Our team is here to help. Whether you’re looking for application guidance, technical specifications, or sample data, we’ll make sure you get the details you need to move forward with confidence. Contact Us
Resources Characterization of Biosimilars Using High-QualityFull-Length Antigens and Second-Generation Biolayer Interferometry
Resources Characterization of Biosimilars Targeting Transmembrane Protein Variants Using Gator Biolayer Interferometry (BLI)