Regen SA (RSA) Kit Regenerable Streptavidin Biosensors and Regeneration Solution Product Number 350009 Product Description The Regen SA (RSA) Kit contains one tray of Gator Regen SA (RSA) Probes with a reusable streptavidin capture surface and the Regeneration Solution for RSA. Buy now Advancing antibody discovery The First Regenerable Streptavidin Biosensor Break free from costly, one-sample-per-sensor workflows with the Gator® Regen SA (RSA) Biosensor. Regenerate, reuse, and reduce cost without compromising performance. Streptavidin biosensors have always been one-and-done. Load your biotinylated target, run the interaction, discard the probe. Regen SA (RSA) probes change that. Built on an engineered streptavidin, RSA regenerates with the same standard protocol you’d use on any reusable capture surface, so a single probe measures up to 10 interactions. Paired with Gator Bio’s XT optics, RSA delivers up to 4x higher signal at up to 8x lower cost per interaction than non-reusable SA sensors. Download Flyer Consistent recapture across a wide dynamic range RSA probes demonstrate robust regeneration performance using the proprietary Regeneration Solution for RSA which effectively removes both the bound ligand and analyte while leaving the streptavidin layer intact. Consistent loading height with recovery of 90-110% is achieved across 10 recapture cycles. 10 recapture cycles of hIgG labeled with biotin-LC-LC on RSA probes at concentrations of 0.625 – 80 μg/mL demonstrating 1.3-5.8% CV on signal loading with 90-110% recovery across all concentrations. Kinetic characterization of biotinylated CRP and PD-L1 with their respective antibodies on the same set of RSA probes with alternating recapture cycles. Capture and screen multiple biotinylated samples with a single reusable biosensor Move through your screening libraries more efficiently by capturing up to 10 different biotinylated samples on the same probe with reliable results. Maintain consistent kinetic performance by using same biosensor for multiple samples. Up to 4x higher signal enabled by XT optics technology XT optics technology increases the signal per bound molecule, improving total signal up to 4x and allowing users to use load fewer ligand molecules to get the same amount of signal as traditional BLI biosensors. This allows users to limit mass transport and avidity effects with ease. Comparison of signal height for CRP/antiCRP kinetics interaction on (A) SA probes and (B) RSA probes. “The Regen SA probes have been an absolute game-changer for our kinetics screening workflows. What we love most is the streamlined, simple workflow; it operates exactly like any other reusable capture assay, allowing us to increase our sample throughput and save us valuable time. We’ve been so impressed that we plan to adopt Regen SA as our primary probe for biotin-tag capture assays.” Sr. Scientist, Commercial Discovery group Key Features Reuse for up to 10 recapture cycleswith consistent loading height performance (≤ 6% CV) Decrease cost per interaction by up to 8xcompared to non-reusable streptavidin biosensors Screen up to 960 samples with a single tray of 96 RSA probes Up to 4x higher signal compared to standard streptavidin sensors Uses a standard regeneration workflow with the kit’s regeneration reagent, maximizing throughput Convenient kit provides all required regeneration reagents and protocols Previous Next Ready to see Regen SA in your lab? Regen SA lets you run hundreds of samples with a single tray of biosensors. Now available in full-tray and trial-size versions so you can try the Regen SA biosensor in your lab right away. Buy now
Anti-Mouse IgG Fc (MFC) XT Probes For quantitation and kinetic characterization of Mouse IgG and Mouse Fc-fusion containing proteins Product Number: 160049 Product Description: A tray of 96 Gator Anti-Mouse IgG Fc (MFC) XT probes coated with anti-Mouse specific capture ligand, for use in Q/K/QKR/EP assays Buy now Advancing antibody discovery The next generation of mouse IgG analysis Achieve faster, more accurate results with high-affinity biosensors that deliver greater binding capacity, broader detection, and cost-efficient reusability for demanding research workflows. Gator® Bio’s new regenerable Anti-Mouse Fc (MFC) XT biosensors provide accurate quantitation and affinity characterization of anti-mouse IgG. Designed for seamless integration into antibody discovery and development workflows, they feature an enhanced design that ensures consistent performance and reproducibility. Download Flyer Binding Kinetics Association and Dissociation Profile of Mouse PD-L1 IgG Binding to Human PD-L1 Previous Next Enhanced sensitivity and dynamic range for mouse antibody screening Gator MFC XT Biosensors are next generation anti-mouse IgG Fc capture sensors engineered for superior performance and broader dynamic range. They support both quantitation and kinetic analysis of mouse IgG antibodies and mouse Fc-fusion proteins with significantly improved sensitivity over previous MFC biosensors. The MFC XT sensors exhibit high affinity for all four mouse IgG subclasses (IgG1, IgG2a, IgG2b, IgG3) with minimal cross-reactivity to rat, human, and bovine IgG. They are suitable for measuring mouse IgG in both crude and purified samples. With up to a 4-fold increase in ligand binding capacity, the sensors provide enhanced kinetic characterization of small proteins and enable detection of low-abundance mouse antibodies in hybridoma cultures. MFC XT Biosensors can be regenerated up to 20 times with minimal loss of performance, delivering a cost-effective solution for high-throughput applications such as lead identification, hybridoma and cell line development, process optimization, and QC testing Download Product Note Faster, more accurate results with high-affinity biosensors Greater binding capacity, broader dynamic range, and cost-efficient reusability for demanding research workflows Rapid quantitation and kinetic analysis of crude and purified mouse IgG Wide dynamic range for fast and accurate quantitation without dilution Improved biosensor surface ligand loading capacity, delivering enhanced sensitivity for kinetics assays Robust and cost effective regeneration, enabling multiple cycles for both kinetics and quantitation applications Key Features Superior mouse IgG specificityHigh specificity to mouse IgG over other species (minimal cross reactivity to rat IgG1) Strong Fc domain bindingStrong binding to Fc domain of all mouse IgG subclasses (Including IgG1, IgG2a, IgG2b, IgG3) Broad dynamic rangeDynamic range: 0.025 – 10,000 μg/mL High quantitation precisionAssay precision: CV <5% Reliable sensor regenerationEasy regeneration with minimal loss of performance Previous Next MFC XT’s higher signal provides better separation, even at high concentrations The MFC XT biosensors deliver significantly higher binding signals compared to competitor sensors, resulting in greater curve separation across a wide concentration range. This improved resolution is especially important at higher concentrations, where competitor data compresses and overlaps, making it difficult to distinguish between samples. With stronger signal and broader dynamic range, MFC XT enables more accurate quantitation and kinetic analysis, even in challenging assay conditions. Low-End Quantitation Assay High-End Quantitation Assay Previous Next