Resources Selection guide for biosensors that bind his-tagged proteins Selection Guide: Gator Probes for His-tagged Protein Capture Gator Bio offers three His-tag capture chemistries, each optimized for different experimental requirements. Selection depends on sample matrix complexity, assay needs, buffer compatibility, and analyte characteristics. Download PDF Recommendations based on sample characteristics: Sample/Assay CharacteristicsAnti-His(PN: 160009)Ni-NTA(PN: 160016)Anti-His (HIS) XT(PN: 160050) Crude samples or complex matrices ✔✔Buffers with reducing agents or chelators✔✔Picomolar affinity measurements✔Small analytes (< 15 kDa) or weak interactions✔Analysis of Fc receptor binding✔✔Sample-limited applications✔Standardized, high-throughput workflows in clean buffers✔✔Table 1: Recommended His-capture probes for common sample and assay needs FeatureAnti-His (Cat# 160009)Ni-NTA (Cat# 160016)Anti-His (HIS) XT (Cat# 160050)Capture SurfaceMonoclonal AntibodyNi-NTA CoordinationAI-Designed NovoBodyTMApplicationsQ / K / EPQ / K / EPQ / K / EPQuantitation Dynamic Range0.25 – 500 µg/ml 0.1 -1000 µg/ml 0.2 – 1000 µg/ml Sample RequirementsHighModerateLowCompatible Sample MatrixPurified proteins E coli lysate CHO Lysate Expi293 Lysate Purified proteins Purified proteins E coli lysate CHO lysate Expi293 lysate Regeneration / Reuse5-10 regenerations 10+ regenerations10+ regenerations Key StrengthsSpecific His-tag recognition; compatible with many buffers Simple, widely used capture chemistry; broad dynamic range Higher signal response per binding event, excellent ligand affinity, low ligand use, optimized to reduce avidity Table 1: Feature comparison between Gator Bio Anti-His, Ni-NTA, and Anti-His (HIS) XT probes. Questions? Our team is here to help. Whether you’re looking for application guidance, technical specifications, or sample data, we’ll make sure you get the details you need to move forward with confidence. Contact Us