The Gator Bio BLI Difference

What Exactly is BLI?

Biolayer interferometry (BLI) technology is at the heart of the Gator Label-Free Systems, enabling highly sensitive measurement of a wide range of small molecules to large protein complexes in both purified and crude matrices. Based on the principle of thin-film interference, BLI compares the interference pattern of white light reflected from an internal reference layer with a layer of immobilized biomolecules on the tip of the biosensor. Shifts in interference due to the accumulation of biomolecules are monitored in real-time to sensitively analyze and calculate rates of association and dissociation among target proteins with high precision. BLI is extremely beneficial for a wide range of applications, because only the interactions of bound biomolecules are measured, excluding the matrix (ie media, buffer, serum) from the interference pattern. 

Do More with BLI

Our specially designed “long walkaway-time” enables users to replace their labor-intensive ELISA methods with a hands-free method for quantitation or other applications. Unlike more complicated methods of measurement, BLI uses reusable biosensors that dip into the well to measure. There are no complicated fluidics to maintain and the system is very robust and maintenance-free.

Benefits for your research


Real-time data



(no complicated fluidics)




Useful for crude or
purified samples


High Sensitivity

(small molecule to large
biomolecular complexes)