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Epitope Binning

Epitope Binning

Knowing the epitope of an antibody is crucial to understanding its biology and therapeutic potential. Running epitope binning analysis earlier in the drug discovery pipeline can save time and increase efficiency.

Gator® BLI can handle crude supernatants to allow epitope binning at the earliest drug discovery phase. Intellectual property rights can be known earlier to make sure you stand out among competitors.

Epitope binning shouldn’t be hard to set up or analyze. With Gator® BLI, assay templates are pre-built to run, and data analysis takes just one click. Whether you’re running the sandwich or tandem format, Gator® can be used to screen your antibodies. Real-time and fluidics-free, the Gator® system enables rapid epitope binning without the difficulties of other label-free methods.

epitope binning

Benefits of Gator Bio Epitope Binning


Rapid assay setup


Single-click data analysis


Rich data sets


Crude or purified samples


What is epitope binning?

Epitope binning tests antibodies in a pairwise manner to determine whether the antibodies compete for the same binding region on an antigen. When many competitions between many antibodies are performed, a set of bins containing those antibodies that compete are created. Antibodies that compete and are in the same ‘bin’ often share a similar function. Choosing antibodies from different bins helps to find new biology and new IP space. Additionally, knowing the bin of an antibody can help predict biological function.

Classical Sandwich

In a classical epitope binning sandwich assay, an antibody is first immobilized on the biosensor surface, followed by the antigen, and then the second antibody. Generally Anti Mouse-Fc or Anti Human Fc biosensors are used for this method.



In a tandem epitope binning assay, the antigen is first immobilized on the biosensor surface, followed by the two antibodies in sequence. Generally, Anti-His or Streptavidin biosensors are used to capture the antigen.