KINETICS
Now, that’s pretty rich
Kinetics
From pM to mM, Gator Bio BLI offers a simple way to do kinetic measurements. Since Gator® BLI displays real-time data, the datasets created enable users to calculate binding kinetics (on-rate and off-rate) and affinity values (dissociation constant).
High-quality, rich data sets reveal information about affinity. Gator® BLI is compatible with a wide range of biomolecules for kinetic investigations: antibodies, nanobodies, small molecules, membrane proteins, AAV, nucleic acids, and more. BLI is a mainstay for the label-free measurement of biomolecular interactions.
Benefits
kon, koff, and Kd in one rapid assay
Wide range of affinity values (mM to pM)
Compatible with crude or purified samples
Label-free method
In contrast to endpoint analyses, BLI data provides a robust data set including the kon, koff, and Kd. The on-rate and off-rate of biomolecular interactions are as important as the resulting affinity. Two biomolecular pairs may have the same affinity value, but vastly different association and dissociation rates. Knowing these differences can help make predictions about activity of biomolecules in vivo.
Specific Applications
- Yes/No binding
- Specificity determination
- GPCR: nanobody interactions
- Off-rate ranking
- PROTAC analysis
- Fc receptors
- Antibody-antigen
- Affinity determination
