Gator® AAV Ratio Kit for Empty/Full Ratio Determination
Gator® AAV Ratio Kit uses the biolayer interferometry (BLI) technology to determine empty (E) versus full (F) content of AAV. It involves three steps: AAV capture using the AAVX probe, lysis for releasing ss DNA, and capture of ss DNA on to single stranded DNA binding (SSB) probe. The ratio is determined by measuring the amount of ss DNA bound to the SSB probes. The method is compatible with crude samples such as cell lysates and cell culture media. The kit brings automation and speed to empty/full ratio measurement, making it easy to integrate into upstream processes.
• AAVX probes (96 probes/tray)
• SSB probes (96 probes/tray)
• Lysis Buffer
• DNA Detection Solution
• AAV Ratio Substrate
• Q Buffer
• SS DNA Standard
• Lysis tubes
5% – 100% full with 10% resolution
8 samples in 40 minutes
24 samples in 120 minutes
Crude Sample Tolerant
• Single technique for empty vs full determination compared to ELISA/ dd PCR
• Crude sample tolerant
• Automated including washes
AAV RATIO KIT WORKFLOW
CAPTURE AND ss DNA MEASUREMENT
Sensogram showing capture of AAV2 capsids with different % full ratios at 3E+11 vp/mL.
Sensogram showing ss DNA binding for standards and samples with different % full ratios.
AAV % FULL STANDARD CURVE
Standard curve for % full ratio using AAV2. This
standard curve is for demonstration purposes only. A standard curve must be run with each assay.