Gator® AAV9 Probes for Rapid and Label-free Quantitation of AAV9
Gator® AAV9 Probes are high specificity, nanobody-based biosensors that enable direct capture and quantitation of adeno-associated virus (AAV) serotype 9 in crude lysates, column eluates, cell lysates, and cell culture supernatants. They serve as an alternative to traditional time-consuming analytical methods such as quantitative polymerase chain reaction (qPCR), droplet digitial polymerase chain reaction (ddPCR), dot blot, and enzyme-linked immunosorbent assay (ELISA). Gator® AAV9 Probes use the proven CaptureSelect™ (Thermo Fisher Scientific) high affinity and high specificity anti-AAV9 nanobody.
AAV9 probes (96 probes/tray)
3 x 109 – 1 x 1013 vp/mL
Assay Run Time
8 samples in 4 minutes
96 samples in 26 minutes
Crude Sample Tolerant
• One-step assay
• Specific for AAV9
• Crude sample tolerant
• Reusable at least 10 times
The graph shows an AAV9 standard curve for concentrations 3.00E+09 to 1.00E+12 (vp/mL). The binding rate between the analyte and the probe surface is a measure of the analyte concentration.
ACCURACY AND PRECISION
The AAV9 serotype was used to test accuracy and reproducibility. The table above shows recovery close to 100% and percent coefficient of variation (%CV) ranging from around 3.70% at medium and high titers to 4.50% at low titers.
AAV9 probes are selective and bind to AAV9 serotype. The figure above shows AAV9 specificity over other serotypes. All serotypes were purchased from www.virovek.com
Performance up to 10 regenerations at 3 concentrations of the same probes with no observed loss in binding rate.