Gator® AAVX Probes for Rapid and Label-free Quantitation of AAV Serotypes
Gator® AAVX Probes are high specificity, nanobody-based biosensors that enable direct capture and quantitation of different serotypes of adeno-associated virus (AAV) in crude lysates, column eluates, cell lysates, and cell culture supernatants, serving as an alternative to traditional time-consuming analytical methods such as quantitative polymerase chain reaction (qPCR), droplet digitial polymerase chain reaction (ddPCR), dot blot, and enzyme-linked immunosorbent assay (ELISA). Gator® AAVX Probes use the proven CaptureSelect™ (Thermo Fisher Scientific) high affinity and high specificity anti-AAVX antibody.
AAVX probes (96 probes/tray)
1 x 109 – 1 x 1013 vp/mL
Assay Run Time
8 samples in 4 minutes
96 samples in 26 minutes
Crude Sample Tolerant
• One-step assay
• Compatible with AAV1-8 and 10
• Broad dynamic range
• Crude sample tolerant
• Reusable at least 10 times
The graph shows an AAV2 standard curve for concentrations 3.90E+09 to 2.5E+11 (vp/mL). The binding rate between the analyte and the probe surface is a measure of the analyte concentration.
ACCURACY AND PRECISION
The AAV5 serotype was used to test accuracy and precision. The table above shows recovery close to 100% and percent coefficient of variation (%CV) ranging from around 1.50% at medium and high titers to 10.90% at low titers.
Binding rate for different AAV serotypes using Gator® AAVX Probes. All serotypes were purchased from www.virovek.com.
Performance up to 10 regenerations at 3 concentrations of the same probes with no observed loss in binding rate.