PALO ALTO, Calif., June 1, 2022 /PRNewswire/ — Gator Bio, the next gen Biolayer Interferometry solutions company, has signed an agreement with I&L Biosystems GmbH, a distributor of innovative biomedical technology and products. The agreement will allow Gator Bio to expand rapidly its product offerings into the European Union, a key region for Gator’s growth strategy as it begins its global reach.

Dr. Hong Tan, CEO of Gator Bio, said, “I&L Biosystems has a history of bringing new and innovative technologies into market — as evidenced by its previous success in commercializing e.g., various multi-analyzer, protein purification devices, SPR instruments, and bioreactors, etc. We look forward to working with the I&L team to bring the Gator platform to users throughout the region, helping meet the demand for therapeutics development and manufacturing.”

“Gator BLI platform and a range of innovative biosensors are an important addition to I&L’s portfolio of protein characterization products, and we are very excited about the possibilities the Gator shall give our customers in characterizing antibodies, AAVs and small molecule drugs,” said Mr. Alexander M. Beljaars, CEO of I&L Biosystems GmbH. “Access to this next gen technology is key to the continued enablement of our biopharma and academia research customers across our geographies. We are very excited about helping research and manufacturing community with the novel applications in gene therapy, immune therapy and lipid nano particle development.”

The latest partnership of two companies builds on Gator Bio’s commitment to providing high-quality BLI platforms globally, recently demonstrated through a strategic partnership with BMS Korea (Bio Medical Sciences) to provide sales and services to biotherapeutics and gene therapy companies in Republic of Korea. To learn more, please visit www.gatorbio.com.

About Gator Bio, Inc.

Gator Bio is a life sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio along with its sister company ET Healthcare are part of Access Medical Systems. The Gator instruments and biosensors enable real-time analysis of biomolecular interactions providing information on affinity, kinetics, concentration and epitope binning, etc. Most importantly, Gator Bio’s analytical capabilities enable better and faster characterization of drug candidates, thus providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China. For more information, please visit www.gatorbio.com.

About I&L Biosystems

I&L Biosystems is a leading distributor of innovative scientific products with the headquarter in Königswinter, Germany. The company was founded in 1991 and has been growing steadily ever since. The focus of I&L is on sales and distribution of high-quality laboratory equipment to customers and researchers in the microbiology, cell biology and biotechnology markets in almost all European countries. High quality of the products, competent advice and fast service are the basis for a long-lasting relationship with our customers and partners in industry, research and development. More than 120 employees ensure that our customers throughout Europe receive excellent support for technical or application-related questions. Visit I&L Biosystems’ website for more information: www.il-biosystems.com.

Media and Investor Relations Contact:
Subodh Nimkar, Marketing Director
[email protected]
+1 650 575 0827
Website: http://www.gatorbio.com//
LinkedIn

PALO ALTO, Calif., Jan. 5, 2022 /PRNewswire/ — Drug makers, biotech companies, biologics developers and CROs will now have the much-needed 21 CFR Part11-compliant software for their GatorPrime and GatorPlus Biolayer Interferometry systems (BLI). The software announced today significantly enhances Gator Bio’s BLI solution for biologics development, which includes application-specific biosensors, Gator^® GatorOne Software and BLI systems.

“Our customers, in no uncertain terms, had told us that Gator Bio’s innovative and high-performance BLI solution would gain wider adoption in their biologics development with 21 CFR Part11 compliant software. Now it is made available. We were committed to delivering this much-needed solution, and with the release of Gator^®  Part11 Software we have delivered on our promise,” said CEO Dr. Hong Tan.

As further proof of our commitment to the Biopharma industry, we are also releasing an IQ/OQ service bundle for GatorPrime and GatorPlus system qualification that will further enhance confidence in data generated using these BLI systems. Both products are ready to ship.

About Gator Bio, Inc.

Gator Bio is a life-sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio and its sister company ET Healthcare are part of Access Medical Systems. Gator instruments and biosensors enable real-time analysis of biomolecular interactions, providing information on affinity, kinetics, concentration, epitope binning, etc. Most importantly, Gator Bio’s analytical capabilities enable better and faster characterization of drug candidates, providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China. For more information, please visit www.gatorbio.com.

Media and Investor Relations Contact:
Subodh Nimkar, Marketing Director
[email protected]
+1 650 575 0827

Gator Bio, Inc. announced the launch of the Gator^TM SMAP probe and Gator^TM mFC probe for GatorPrime and GatorPlus biolayer interferometry (BLI) systems today. 

The company has been focused on the development and commercialization of the novel biosensors for the next generation BLI that greatly enhances the performance and cost effectiveness of this simple yet powerful analytical technology. 

“We saw gaps and challenges in drug discovery and development using BLI technology and decided to develop new surface chemistries for the probes,” said Dr. Hong Tan, CEO of Gator Bio. “We believe these new probes will significantly reduce the cost and enhance biopharma research capabilities.” 

The Gator^TM SMAP probe designed for small molecule-protein binding studies has demonstrated significantly improved sensitivity. The Gator beta users also demonstrate best-in-class performance for small peptide kinetic screening against full length of antibodies that are generally difficult to perform using traditional BLI. 

The typical antibody screening from hybridomas commonly utilizes ELISA technology. The Gator^TM mFC probe enables very high sensitivity, 5-log dynamic range, and provides a much simpler and quicker workflow than ELISA. The unique capability of regeneration up to 20 times or more without performance degradation provides a highly cost-effective solution for biopharma.

Detailed info on the Gator^TM SMAP probe and Gator^TM mFC probe along with Gator Bio’s complete portfolio of products can be found here. 

These biosensors come on the heels of the recent release of Gator^TM Flex SA Kit and Gator^TM AAVX probes.

About Gator Bio, Inc.

Gator Bio is a life sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio along with its sister company ET Healthcare are part of Access Medical Systems. The Gator instruments and biosensors enable real-time analysis of biomolecular interactions providing information on affinity, kinetics, concentration, and epitope binning, etc. Most importantly, Gator Bio’s analytical capabilities enable better and faster characterization of drug candidates, thus providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China. For more information, please visit www.gatorbio.com.

SOURCE Gator Bio, Inc.

Media and Investor Relations Contact:

Subodh Nimkar, Marketing Director 

[email protected]

+1 650 575 0827

Web site: https://www.gatorbio.com//

LinkedIn

PALO ALTO, Calif., Sept. 14, 2021 /PRNewswire/ — Gator Bio, Inc. announced today the launch of the GatorPlus, a next generation biolayer interferometry (BLI) instrument and two new biosensor products, Gator™ Flex SA Kit and Gator™ AAVX probe. The GatorPlus adds to the currently available GatorPrime instrument; and the new Gator™ Flex SA Kit and Gator™ AAVX probe expand the off the shelf biosensor portfolio to 14 products for the North America market. Gator Bio also offers on demand custom biosensors for specific application needs.

GatorPlus is a bench-top instrument for real-time label-free analysis of molecular interactions and quantitation in 96-well or 384-well microplates. The instrument offers longer walk-away time and more automation compared to GatorPrime, the first Gator instrument launched in March 2019.     

Based on Gator Bio’s proprietary technology, the Gator™ Flex SA Kit is the industry’s first ever reactivable streptavidin biosensor for BLI. It can be reactivated and reused more than 20 times without performance degradation, enabling significant cost saving for Gator system customers.

The Gator™ AAVX probe quantitates many serotypes of AAVs automatically in minutes, greatly simplifying AAV developers’ workflow. The results obtained from the Gator™ AAVX probes correlate tightly to the gold standard method.

Gator Bio was founded by Dr. Hong Tan and Mr. Robert Zuk, the pioneers of biolayer interferometry. The company has been focused on development and commercialization of the next generation BLI that greatly enhances the performance and cost effectiveness of this simple yet powerful analytical technology.

In addition to the newly introduced Gator™ Flex SA Kit and Gator™ AAVX probes, Gator Bio offers a wide range of products, including the highly sensitive SMAP biosensor and the regenerable mouse Fc capture biosensor. The Gator systems and associated biosensors provide convenient kinetic analysis and concentration determination for the development of antibody and protein therapeutics.

“We saw problems and shortcomings with the original BLI technology, so we decided to come back to close the gaps with new ways to realize BLI” said Dr. Hong Tan, CEO of Gator Bio. “Our objective is to enable the most effective and efficient BLI systems for scientists. “

Detailed info on the Gator™ Flex SA Kit and Gator™ AAVX probes along with Gator Bio’s complete portfolio of products can be found here. You can also hear inventors talk about the technology and the Gator Bio’s vision.

About Gator Bio, Inc.

Gator Bio is a life sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio along with its sister company ET Healthcare are part of Access Medical Systems. The Gator instruments and biosensors enable real-time analysis of biomolecular interactions providing information on affinity, kinetics, concentration and epitope binning, etc. Most importantly, Gator Bio’s analytical capabilities enable better and faster characterization of drug candidates, thus providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China. For more information, please visit www.gatorbio.com.

Media and Investor Relations Contact:
Subodh Nimkar, Marketing Director
[email protected]
+1 650 575 827

Introduction

Many of the label-free biosensors have been regenerated successfully to reduce cost for drug research and discovery. However, extensive application of streptavidin biosensors is still hindered by the irreversible immobilization of the bait molecule, and the ability to use the same probe for different application is desirable for cost effective research.

Gator Flex SA Kit

Gator^® Bio’s Gator Flex SA Kit is the first in the market to provide reactivable streptavidin probes with a reagent set. One probe can easily be used by different users with different applications, which makes it very useful for academics or for use in fully automated screening of different biomolecular interactions. The Gator™ platform enables researcher to monitor whole reactivation process rapidly and provides flexibility and confidence.

Performance Summary

• Remove both capture reagent and biotin-bait molecule and immobilize new capture reagent < 5 minutes 
• Use with same or different biotinylated bait 
• Can be re-used 10 times or more without loss of performance 
• Automated reactivation 
• Reagents can be stored at 4°C for up to 5 months 

Results

Loading height reproducibility

Three Flex SA probes were tested over 10 reactivations using biotinylated TNF-α at 1µg/mL. The table below shows CV < 5.0% suggesting excellent loading height reproducibility.

Reproducibility of K_D over 10 reactivations using the same bait 

Kinetics was evaluated using biotinylated TNFα and anti-TNFα antibody using a 5 min 1000 rpm pre-wet in K buffer and capture of Flex SA Capture Reagent (SA-CR). The biotinylated TNF-α was loaded onto the sensor, then exposed to association and dissociation of 10, 30, 100 and 300 nM anti TNF-α antibody. Global-fit analysis using Gator Bio software for the TNF-α binding interaction with anti- TNF-α resulted in KD = 7.63E-10 M. 

K_D measurements for different proteins over 10 reactivations 

The same probe was tested with 2 different proteins over 10 reactivations. A CV < 5% was observed for both proteins. 

Gator Bio Total Solution 

Gator^TM Flex SA kit

Gator^TM Prime or Gator^TM Plus 

Gator Bio Software 

Ordering info 

PL168-350001: GatorTM Flex SA Kit 

Introduction

Adeno associated virus (AAV) capsids are a leading modality for in vivo gene delivery. Complete and precise characterization of capsid particles, including capsid and vector genome concentration, is necessary to safely and efficaciously dose patients. In virus development and production, it is important to determine the virus concentration at different stages of the process, to optimize the clone used as well as the production yields. Biolayer Interferometry (BLI) using AAV specific biosensors is a rapid label-free method for quantitation of AAV serotypes. 

Gator AAVX Probes 

The Gator AAVX probes are a high specificity antibody-based biosensors that enable direct capture and quantitation of different serotypes of AAV in crude lysates, column eluates, cell lysates and cell culture supernatants, serving as an alternative to traditional time-consuming analytical methods, such as qPCR, ddPCR, Dot blot and ELISA. The Gator AAVX probe uses proven CaptureSelect™ (Thermo Fisher Scientific) high affinity and high specificity anti-AAVX antibody. 

Performance Summary 

• Dynamic range: 1 x 109–1 x 1013 vp/mL for most AAV serotypes 
• Throughput: 8 samples in 10 minutes, 96 samples in 120 minutes
• Limit of detection: typically, 5 x 108 vp/mL (serotype dependent)
• Crude sample tolerant
• Stable over broad pH range 
• Cost effective – Reusable at least 10 times by regeneration 

Results 

Dynamic range: 

The AAV2 serotype range was tested using the Gator AAVX probes. The data shows 4 orders of magnitude dynamic range. The data was acquired using 1:3 dilution in 10 min at 1,000rpm. 

Accuracy and reproducibility 

AAV5 serotype was used to test the accuracy and reproducibility. The table below shows recovery close to 100% and CV ranging from around 1.5% at medium and high titers to 10.9% at low titer. 

Binding rate of different AAV serotypes 

The binding rate of 8 different serotypes of AAV were studied. The figure below shows binding rates of tested serotypes at 2 x 1011 vp/mL concentration. 

Regeneration performance 

Data below shows performance after 10 regenerations of the same probe. No loss in binding rate observed even after 10 regenerations 

Gator Bio Total Solution 

Gator^TM AAVX Probes

Gator^TM Prime OR Gator^TM Plus

Gator Bio Software

Ordering info 

PL168-160017: GatorTM AAVX Probe